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10 results

Publishedv1.0Personal protocol

Single-Color Compensation Beads

Núria Crua·In vitro · Flow Cytometry / FACS · Synthetic Sample·Updated Jul 31, 2026

Reproducible

30 min 1 reagentsReagents & materialsCompensation BeadsBiolegend · 42460100

Publishedv1.0Personal protocol

Isolation of Mouse Bone Marrow Cells for BMDM Differentiation

Núria Crua·In vitro · Ex vivo · Mouse·Updated Jul 28, 2026

Reproducible

3 reagentsReagents & materialsPenicillin/StreptomycinTGF-βGM-CSF00

Publishedv1.0Personal protocol

Bacterial killing assay protocol

Núria Crua·In vitro · Bacteria · Cell Culture·Updated Jul 28, 2026

Reproducible

4540 min 5 reagentsReagents & materialsddH2OPBS (Phosphate Buffered Saline)RPMI + FCS (Fetal Calf Serum)Gentamycin0.1% NP40 in PBS00

Publishedv1.2Personal protocol

Macrophage Isolation from Lung Tissue

Núria Crua·Ex vivo · Mouse · Flow Cytometry / FACS·Updated Jul 27, 2026

381

105 min20

Publishedv1.0Personal protocol

RNA delivery into Primary Human Neutrophils by Electroporation

Núria Crua·Ex vivo · Primary Cells · Human·Updated Jul 27, 2026

211

81 min00

Publishedv1.3Personal protocol

Immunofluorescent HeLa cells

Núria Crua·In vitro · Cell Lines · Human·Updated Jul 28, 2026

Reproducible

315 min 11 reagentsReagents & materialsProlong GlassInvitrogen · P36934Phosphate buffered saline (PBS) (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.76 mM KH2PO4, pH 7.4)Tris buffered saline (TBS) (50 mM Tris, 150 mM NaCl, pH 7.4)Fixation reagent: 4% Formaldehyde in PBSPermeabilization reagent: 1% Triton X-100 in 1x PBSBlocking reagent: 10% normal serum (Sigma H4522), 0.1% Triton X-100 in PBS+5 more31

Publishedv1.0Personal protocol

RIPA-Based Total Protein Extraction from Mammalian Cells

Núria Crua·In vitro · Primary Cells · Cell Lines·Updated Jul 27, 2026

Reproducible

40 min01

Publishedv1.1Personal protocol

Minimum Inhibitory Concentration (MIC) by Broth Microdilution

Núria Crua·In vitro · Bacteria · Antibiotics·Updated Jul 27, 2026

Reproducible

11

Publishedv1.1Personal protocol

E. coli Competent Cell Preparation and Transformation

Núria Crua·In vitro · Bacteria · DNA·Updated Jul 27, 2026

Reproducible

119 min 4 reagentsReagents & materialsE. coli colony or glycerol stockLB (Luria-Bertani) medium0.1 M CaCl2, autoclavedPlasmid DNA (approx. 50-100 ng/µl)11

Publishedv1.1Personal protocol

Thawing of THP-1 Cell Line

Núria Crua·In vitro · Cell Lines · Human·Updated Jul 27, 2026

Reproducible

35 min 4 reagentsReagents & materialsRPMI 1640 culture mediumFetal bovine serum (heat-inactivated), 10%Antibiotic (Penicillin/Streptomycin/Amphotericin or Gentamicin), 1%DMSO (used for cryopreservation)11

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