JNAR Protocol

E. coli Competent Cell Preparation and Transformation

Núria Crua· Postdoc· University of Lausanne (UNIL)

Published Jul 27, 2026

~1h 59m total· 1 contributions

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JNAR-ID: JNAR-69755530

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PublishedLast updated Jul 27, 2026

Objective

Preparation of chemically competent E. coli cells using calcium chloride and their subsequent transformation with plasmid DNA.

Classification

Sample typeDNA

TechniqueCloningTransfection

Experimental contextIn vitro

Biological modelBacteria

Reproducibility status

Reproducible

No reproducibility blockers or risks reported. The protocol runs end-to-end.

20 healthy

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Núria Crua · Jul 27, 2026

Integrated