Núria Crua· Postdoc· University of Lausanne (UNIL)
Published Jul 27, 2026~1h 45m total· 2 contributions
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JNAR-ID: JNAR-91788110
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PublishedLast updated Jul 27, 2026
Objective
To isolate lung macrophages from mouse lung tissue using enzymatic digestion, Percoll gradient separation, magnetic bead-based enrichment with anti-F4/80 microbeads.
Classification
Sample typeTissue
TechniqueFlow Cytometry / FACS
Experimental contextEx vivo
Biological modelMouse
Reproducibility status
Not reproducible
1 step blocks the protocol — it can't be completed end-to-end until it is fixed.
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